Working under general supervision, the qualified Research Assistant 2 is responsible for the conduct of research projects in the field of Cancer biology (specifically melanoma, skin cancer). This includes data analysis, and oral and written presentation of the results to the lab members. The research assistant will work on the research projects related to uncovering the mechanisms of chemotherapy resistance and identifying the novel biomarkers that can assess the drug efficacy in melanoma using in vitro and in vivo models. The position will work with animals.
- Plan and carry out in vitro and in vivo animal experiments for various research projects in accordance with general plans related to uncovering the mechanisms of chemotherapy resistance and identifying the novel biomarkers that can assess the drug efficacy in melanoma using in vitro and in vivo models. (7%)
- Conduct a series of experiments to supply scientific information as part of a larger project. conduct cell culture of established and primary cell lines, cell proliferation, clonogenic survival, DNA/RNA extraction, immunoblot, PCR, plasmid cloning and purification, maintain laboratory reagents including buffers and medias, and measure weight and tumor size in control and experimental mice. (53%)
- Assist in developing improved techniques, projection methods or procedures in selection of cell lines, authentication, metabolic aspects on melanoma to entail optimization of established protocols. (7%)
- Collect and analyze data, prepare graphs, charts and slides from experimental data for purposes of reporting. Perform analysis which may include specific quantitation using ELISA-based methods. Maintain a database tracking biospecimens. (7%)
- Maintain certain equipment facilities. (7%)
- Maintain chemical/biological inventory in compliance with EHS. Assess new equipment that may be purchased by the lab.
- Oversee inventory and order supplies necessary for daily laboratory work. (7%)
- Train new staff including undergrad students regarding standard laboratory policies as well as basic biochemistry research techniques. (7%)
Perform other duties as assigned (5%).
Department: Daily contact with supervisor to discuss research and maintain workflow.
University: Occasional contact with other departments to share information.
External: Limited or no contact with vendors to exchange information.
Students: Contact with student employees to exchange information.
This position has no direct supervision of staff employees. Train new staff; direct the work of students.
Education and experience: bachelor's degree and 1 to 3 years of related experience or associate's degree in an approved biotechnology program and 2 to 4 years of experience.
- Has knowledge of commonly-used concepts, practices, and procedures within molecular biology and tissue culture skills (PCR, Immunoblot, Retroviral/Lentiviral production).
- Relies on instructions and pre-established guidelines to perform the functions of the job.
- Ability to operate laboratory equipment.
- Ability to meet consistent attendance.
- Ability to interact with colleagues, supervisors and customers face to face.
- Previous experience working with animals preferred.
- Must demonstrate compliance with university animal research and care (ARC) policies and procedures and compliance to regulations of Animal Welfare Act, Public Health Service Policy, AAALAC guidelines and other applicable regulatory guidelines.
- Must demonstrate compassion for animals within university facilities and dedication to the Animal Resource Center's mission. Must handle animals with care and respect at all times.
- Must be able and willing to learn new techniques, procedures, processes, and computer gear to protect the health of the animals.
General laboratory environment. The lab is open floor plan with abundant bench space for animal handling and manipulations. Ample desk space with computers are also provided. The lab is equipped with one shared fume hood for storage of hazardous and non-hazardous materials. May have exposure to hazards.
Case Western Reserve University's animal facilities are accredited by the Association for the Assessment and Accreditation of Laboratory Animal Care (AAALAC) and is managed according to the "Guide for the Care and Use of Laboratory Animals appropriate Federal Animal Welfare Regulations, and the Public Health Service Policy on the Humane Care and Use of Laboratory Animals." This position, and all animal research personnel, are subject to internal compliance to SOM Animal Resource Center Standard Operating Procedures and to compliance regulations of the Animal Welfare Act, Public Health Service Policy, AAALAC guidelines, the State of Ohio Veterinary Practice Act, Federal Drug Enforcement Administration regulatory guidelines, US Food and Drug Administration Center for Veterinary Medicine regulations and other applicable regulatory guidelines.